Carl Sagan opens the program with a description of the cosmos and the Spaceship of the Imagination. The ship journeys through the universe's hundred billion galaxies, the Local Group, the Andromeda Galaxy, the Milky Way, the Orion Nebula, our Solar System, and finally the planet Earth. Eratosthenes' successful calculation of the circumference of Earth leads to a description of the ancient Library of Alexandria. Finally, the Ages of Science are described, before pulling back to the full span of the Cosmic Calendar.

20 years ago, two astronomers made a remarkable discovery, one which would change the way we view the universe for ever. A planet outside our solar system, orbiting a distant star - an exoplanet. Since then, we have found worlds where it rains diamonds, ones that boil at 3,000 degrees centigrade and even a world with four suns in its sky. But the big question is - will we ever find another Earth? As we close in on the discovery of the 2,000th planet outside our solar system, or exoplanet, we investigates the techniques that are revealing so much about these alien worlds. The documentary asks if we are really any closer to finding another world like our own - a second Earth.

Famed astrophysicist Neil deGrasse Tyson takes a tour of the Solar System and the known universe establishing the components of Earth's "address" within the Virgo Supercluster. He then shares the story of the person who championed an expansive understanding of Earth's place in the universe by presenting Renaissance Italian Giordano Bruno's vision of the universe as a limitless expanse of space and time. He then makes an exploration into the Cosmic Calendar, which dates back to the dawn of the Big Bang (similar to the presentation from episode 1 of the original series). The episode ends with deGrasse Tyson narrating how he met his mentor Carl Sagan, who hosted the first Cosmos series.

This episode covers the nature of how life may have developed on Earth and the possibility of life on other planets. Tyson begins by explaining how the human development of writing systems enabled the transfer of information through generations, describing how Princess Enheduanna ca. 2280 BCE would be one of the first to sign her name to her works, and how Gilgamesh collected stories, including that of Utnapishtim documenting a great flood comparable to the story of Noah's Ark. Tyson explains how DNA similarly records information to propagate life, and postulates theories of how DNA originated on Earth, including evolution from a shallow tide pool, or from the ejecta of meteor collisions from other planets. In the latter case, Tyson explains how comparing the composition of the Nakhla meteorite in 1911 to results collected by the Viking program demonstrated that material from Mars could transit to Earth, and the ability of some microbes to survive the harsh conditions of space. With the motions of solar systems through the galaxy over billions of years, life could conceivably propagate from planet to planet in the same manner. Tyson then moves on to consider if life on other planets could exist. He explains how Project Diana performed in the 1960s showed that radio waves are able to travel in space, and that all of humanity's broadcast signals continue to radiate into space from our planet. Tyson notes that projects have since looked for similar signals potentially emanating from other solar systems. Tyson then explains that the development and lifespan of extraterrestrial civilizations must be considered for such detection to be realized. He notes that civilizations can be wiped out by cosmic events like supernovae, natural disasters such as the Toba disaster, or even self-destruct through war or other means, making probability estimates difficult. Tyson describes how elliptical galaxies, in which some of the oldest red dwarf stars exist, would offer the best chance of finding established civilizations. Tyson concludes that human intelligence properly applied should allow our species to avoid such disasters and enable us to migrate beyond the Earth before the Sun's eventual transformation into a red giant.

Travel from the inner solar system to the Kuiper Belt and explore the moons surrounding the planets of the solar system. Many of these moons that were once unknown are now on the cutting edge of astronomical study. Some burst with volcanic fury another spews icy geysers and others offer the possibility of alien life. Are these strange worlds simply hostile environments unfit for humans or do other possibilities exist? Cutting-edge computer graphics are used to bring the universe down to earth and to imagine what kind of life forms might evolve in alien atmospheres.

Ideas about time and space are explored in the changes that constellations undergo over time, the redshift and blue shift measured in interstellar objects, time dilation in Albert Einstein's theory of relativity, the designs of both Leonardo da Vinci and spacecraft that could travel near light speed, time travel and its hypothetical effects on human history, the origins of the Solar System, the history of life, and the immensity of space. In Cosmos Update, the idea of faster-than-light travel by wormholes (researched by Kip Thorne and shown in Sagan’s novel Contact) is discussed.